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KMID : 0363420140280030047
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Journal of Korean Oriental Pediatrics
2014 Volume.28 No. 3 p.47 ~ p.58
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Anti-inflammatory Activities of GyejigaChulBuTang on Lipopolysaccharide-stimulated RAW264.7 Cells
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Jeong Ju-Hui
Lee Seung-Yeon
Yu Sun-Ae
Kang Kyung-Hwa
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Abstract
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Objectives: GyejigaChulBuTang (GCBT) is a prescription used to treat acute and chronic arthritis in Korea, China, and
Japan. This study assessed the anti-inflammatory and anti-oxidant activities of GCBT on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells.
Methods: Raw264.7 cells were pretreated with or without GCBT for 1 hour prior to incubation with LPS. Anti-inflammatory activity of GCBT was evaluated with reference to gene expression and production levels of proinflammatory cytokines (TNFα, IL-1β, IL-6, GM-CSF and INFγ) and inflammatory mediators (iNOS, COX-2, NO and PGE2). In addition, intracellular ROS generation and signal transduction of MAPK family, PI3K/Akt and IκBα/NFκB was investigated.
Results: Prior treatment with GCBT inhibited elevation of TNFα, IL-1β, IL-6, GM-CSF, INFγ, NO and PGE2, together with their cognate mRNAs in a dose-dependent manner. Intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of MAPK family, PI3K/Akt and IκBα as well as nuclear translocation of NFκB.
Conclusions: GCBT suppresses pro-inflammatory mediators. GCBT has potential in the treatment of juvenile rheumatoid
arthritis associated with inflammation.
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KEYWORD
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GyejigaChulBuTang, RAW264.7 cells , Proinflammatory cytokine, Juvenile rheumatoid arthritis, Inflammation
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